|
KMID : 0624620080410050394
|
|
BMB Reports
2008 Volume.41 No. 5 p.394 ~ p.399
|
|
|
Intrinsic bent DNA colocalizes with the sequence involved in the Nd-sD mutation in the Bombyx mori fibroin light chain gene
|
|
Barbosa Joice Felipes
Bravo Juliana Pereira
Takeda Karen Izumi
Zanatta Daniela Bertolini
Silva Jose Luis da Conceicao
Balani Valerio Americo
Fiorini Adriana
Fernandez Maria Aparecida
|
|
|
Abstract
|
|
|
|
Multiple sequence alignments of the Bombyx mori fibroin light chain gene (fib-L) from hybrids and from Chinese and Japanese strains demonstrated that 51.6% of the fib-L third intron is conserved. One of these conserved segments, 41 bp long, contains the sequence CGTTATTATACATATT, which is duplicated in the B. mori Nd-sD mutant. In the present work, electrophoretic mobility assays and computational analyses revealed a major peak of intrinsic bent DNA within the segment that undergoes breakage in the previously-described Nd-sD mutation. This result suggested that this intrinsically-curved region might mediate DNA cleavage and enhance recombination events in the third intron of the Bombyx mori fib-L gene. [BMB reports 2008; 41(5): 394-399]
|
|
|
KEYWORD
|
|
|
Bombyx mori, fib-L third intron, Fibroin light chain gene, Intrinsic bent DNA sites, Nd-sD mutation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|